Tricoloma matsutake mycelium growth promoting fungi and its species abundance in fungal community within fairy ring

Despite Tricholoma matsutake has been favorite food in human societies, every attempts to artificially cultivating this favorite food have been failed. As an ectomycorrhizal fungus, Tricholoma matsutake has specific habitats named fairy ring which has closed relationship with the host plant Pinus densiflora. For the artificial cultivation, contribution of biological factors such as microbial communities in T. matsutake habitats is required. In this study, we collected 461 fungal isolates from T. matsutake habitat in yangyang-gun, south Korea. From the fungi, filtered metabolites were obtained and among them, 4 fungal metabolites showed growth promoting effect on T. matsutake mycelium. The 4 fungal isolates were analyzed by using ITS region sequencing and identified as Penicillium, Umbelopsis, and Aspergillus. In addition, pyrosequencing method was performed to analyze the characteristics of fungal communities from fairy ring. In 2022 and 2023, we collected soil core samples from the same T. matsutake habitat on May and September, and total 623,324 species and 826,124 species were identified, respectively. Changes of composition in fungal community were compared between the spot where the T. matsutake developed and the spot where did not. In the comparison process, the abundance of fungal isolates which promoted the growth of T. matsutake mycelium were also analyzed to identify the effect of the fungal isolates on T. matsutake development. Through the result, it is expected to ultimately contribute to the creation of T. matsutake development prediction models.