MTT Toxicity Assessment According to Mushroom Extract Addition Ratio

MTT Toxicity Assessment According to Mushroom Extract Addition Ratio

Eun-woo Cho1, Min-Seo Jung1, Yu-Bin Park1, Tae-Min Park1, 

Youn-Jin Park2, Myoung-Jun Jang1,3*

1Department of Plant Resources, Kongju National University,

2Kongju National University Legumes Green Manure Resource Center

3Center for Smart Future Agricultural Education and Research, College of Industrial Sciences, Kongju National University

*Email: plant119@kongju.ac.kr

This study prepared extracts of I. lacteus (Irpex lacteus), P. pulmonarius (Pleurotus pulmonarius), B. fumosa (Bjerkandera fumosa), and G. sp. (Ganoderma sp.) at 100 mg/mL and used them for an MTT assay. As a result, when each mycelial extract was applied at a 1% concentration, I. lacteus was 102.5%, P. pulmonarius was 92.7%, B. fumosa was 102.6%, and G. sp. was 102.3%. At 3%, I. lacteus was 102.3%, P. pulmonarius was 92.4%, B. fumosa was 102.5%, and G. sp. was 102.1%. At 6%, I. lacteus was 102.1%, P. pulmonarius was 92.1%, B. fumosa was 102.2%, and G. sp. was 95.5%. At 9%, I. lacteus was 102.0%, P. pulmonarius was 92.0%, B. fumosa was 102.0%, and G. sp. was 95.1%. Based on these results, when RAW 264.7 cells were treated with the mycelial extracts at 0–9%, all treatment groups-maintained cell viability above 90%, indicating no cytotoxicity in RAW 264.7 cells. Therefore, this study is expected to provide basic in vitro safety information to suggest the potential use of these four mycelial extracts as food ingredients