English
Growth & Protein of Irpex lacteus, Bjerkandera fumosa with
Carbon and Nitrogen Supplementation
Jun-Hyung Song1, Dong-Ryeol Yu1, Yu-Bin Park1, Ju-Young Park1,
Youn-Jin Park2, Myoung-Jun Jang1,3*
1Department of Plant Resources, Kongju National University,
2Kongju National University Legumes Green Manure Resource Center
3Center for Smart Future Agricultural Education and Research, College of Industrial Sciences, Kongju National University
*Email: plant119@kongju.ac.kr
This preliminary study was conducted as a foundational experiment to produce mycelium-based meat analogues. The strains used were Irpex lacteus and Bjerkandera fumosa. The control medium was potato dextrose broth (PDB), and the treatment media were PDB supplemented with five carbon sources and three nitrogen sources, each at 2% (w/w) relative to the medium weight. Mycelial growth and total protein were analyzed. In I. lacteus, fresh weight among the carbon-source treatments did not differ from the control except for glucose, whereas all nitrogen sources increased growth. In the skim-milk medium, dry weight approximately doubled, and total protein increased 1.7-fold with dextrin and threefold with skim milk. In B. fumosa, fresh weight increased 1.3-fold with dextrin and with skim milk, and dry weight increased 1.8-fold with skim milk. Total protein decreased in most treatments relative to the control but increased fourfold with skim milk. Taken together, skim milk most effectively enhanced growth and protein metrics in both strains, whereas dextrin enhanced protein content in I. lacteus. These results provided a rationale for prioritizing skim milk as a supplementary component in medium design for the development of mycelium-based meat. This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry(IPET) through High Value-added Food Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs(MAFRA)(RS-2024-00403514)
