English
Phylogenetic relationship analysis and comparison of the taxonomic positions of four Sanghuang mushroom-derived isolates based on ITS sequences
Jun Hyung Song1, Yoon Seo Jang1 Patrícia Harčárová4 ,Min Gyeong Kang1, Tae Min Park1, Tae Kwon Kim1,Tae Seok Oh1, Youn Jin Park2, Myoung Jun Jang1,3*
1Department of Plant Resources, Kongju National University,
2Kongju National University Legumes Green Manure Resource Center
3Center for Smart Future Agricultural Education and Research, College of Industrial Sciences, Kongju National University
4Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic
*Email: plant119@kongju.ac.kr
To determine the taxonomic positions of Sanghuang mushroom-derived isolates, ITS sequence-based phylogenetic analyses were conducted for isolates Si2, Si4, Si7, and Si8. Consensus sequences were generated by combining ITS1 and ITS4 reads for each isolate, and phylogenetic trees were constructed using reference sequences obtained from GenBank by the neighbor-joining method with bootstrap analysis. Si2 showed high sequence similarity to Phellinus igniarius; however, it did not cluster stably within the P. igniariusreference clade and instead formed an independent branch in the ITS tree. Si4 and Si7 were clearly separated from the Sanghuangporus sanghuangand Phellinus linteusgroups and were positioned closer to the P. igniarius–P. alnilineage, although neither isolate was stably included within a single species-level reference clade. In contrast, Si8 was separated from the P. igniarius–P. alnilineage and was placed close to the Sanghuangporus sanghuangreference sequences, suggesting that it most likely belonged to the S. sanghuanglineage. These results indicated that ITS sequence-based analysis was useful for estimating the overall phylogenetic positions of Sanghuang mushroom-derived isolates, but also had limitations for species-level identification of some isolates. Therefore, additional analyses using supplementary genetic markers and carefully selected reference sequences were considered necessary for more reliable taxonomic identification.
