English
High-Throughput Phenotyping Systems for Resistance Screening to Brown Spot and Sheath Blight in Long-Grain Rice
Chawoon Kang1,2, Seoyeon Kim1,2, Seol-Hwa Jang1, and Sook-Young Park1,2
1Department of Plant Medicine, Sunchon National University, Suncheon 57922, Korea
2Interdisciplinary Program in IT-Bio Convergence System (BK21 plus), Sunchon National University, Suncheon 57922, Korea
*Email: spark@scnu.ac.kr
Rice production is increasingly threatened by fungal diseases whose severity has intensified under changing climate conditions. To improve resistance screening efficiency, high-throughput phenotyping systems were developed for two major rice diseases: brown spot and sheath blight. Brown spot, caused by Bipolaris oryzae, develops rapidly under high temperature and humidity but produces small, scattered lesions that are difficult to quantify manually. An automated image-based analysis system was therefore developed to measure lesion area percentage with 90–100% precision. Using this platform, approximately 150 long-grain rice accessions were screened for resistance. For sheath blight, caused by Rhizoctonia spp., a standardized inoculation method was established by inoculating the basal sheath with 14-day-old cultures and incubating plants at 31 °C and 100% relative humidity. Lesions developed within 4 days and expanded upward to 15–20 cm by 10 days after inoculation, allowing reliable disease severity assessment. These phenotyping systems provide efficient tools for evaluating resistance to major fungal diseases and facilitate the identification of resistant germplasm in long-grain rice. (This work was supported by the Rural Development Administration of Korea (RS-2025-02214096))
