English
Isolation and Characterization of Antibacterial Secondary Metabolites from Freshwater-Derived Trichoderma flavipes
Jeong Tae Kim, Yun Jeong Heo, Gil Han, Sanghee Lee, Hye Yeon Mun, Hye Jin Hwang, YunJi Cho, Chang Soo Lee and Jaeduk Goh.
Research Department, Nakdonggang National Institute of Biological Resources, Sangju 37242, Republic of Korea.
Freshwater-derived fungi are considered promising sources of bioactive secondary metabolites. In this study, Trichoderma flavipes isolated from a freshwater environment was investigated for its antibacterial potential. The crude extract of the fungal culture exhibited notable antibacterial activity against Gram-positive bacteria, including Staphylococcus aureus and Bacillus cereus. To identify the bioactive constituents responsible for this activity, the culture extract was subjected to chromatographic separation. Purification using high-performance liquid chromatography (HPLC) led to the isolation of five secondary metabolites. The structures of the isolated compounds were elucidated by nuclear magnetic resonance (NMR) spectroscopy. Bioactivity assays revealed that some of the isolated compounds showed inhibitory activity against S. aureus and B. cereus. These findings highlight freshwater-derived T. flavipes as a potential source of antibacterial secondary metabolites and provide a basis for further investigation of its bioactive compounds.
